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Spermiology Diagnostic Image Spermogramme
EQA 2023 - Spermology
Expert Biologists - Scheme Coordinators:
André FORCE, Marie LETY, Marie LOPEZ and Benoit SCHUBERT
EQA performed since 2018
Number of participating laboratories in 2022: 33

1 – Scheme overview

The scheme is an external quality assessment scheme for semen analysis tests, developed in collaboration with the ARBRE association (Association Rhônalpine des Biologistes de la Reproduction). It provides global assessment of seminograms and cytological semen analysis. It covers 4 parameters:

  • Sperm count: using a sample of human semen
  • Round cells count: using a sample of human semen
  • Vitality: digitalized smear (eosin – nigrosin) available on our site
  • Morphology: digitalized smear (sperm stain) available on our site
  • Motility: observed on a on-line video on our site

The clinical context is provided with each sample/smear as well as complementary clinical results (pH, etc.) so that you can propose a diagnostic hypothesis based on your results.

INFORMATION: A poster was presented during the 23rd days of the FFER, in September 2018: "EQA in Spermiology: a new scheme for a overall assessment of the exam"
==> Download the PDF (in french)

2 – Assessment frequency

Number of investigations per year : 4
Number of control samples per investigation: 1 semen sample, 2 digitalized smears and 1 video
Number of control samples per year: 1 semen sample, 8 digitalized smears and 1 video

3 – List of tests assessed


  • Sperm count
  • Round cells count (1 survey / year)
  • Vitality
  • Motility

Cytological semen analysis :

  • Morphology

Following examination, 3 conclusions are provided:

  • Conclusion about the seminogram
  • Conclusion about the cytological semen analysis
  • Global conclusion taking into account other clinical findings

4 – Description of the control samples

Liquid human control semen (ready-to-use, 0,5 mL samples).

Virtual media: Digitalized smears + Videos

5 - Clinical setting

Clinical setting are proposed in each investigation .

6 - Computer Prerequisites

Panneau attention COMPUTER PREREQUISITES Panneau attention
Central Unit: Core i5 or equivalent, 2 GB RAM Windows XP SP3
or 4 GB RAM Windows 7 and higher
Browser: Internet Explorer 10 to 11, Firefox, Chrome, Microsoft Edge
Resolution: 1400 x 900 minimum, 1920 x 1080 optimal
Minimum speed: 8 Mbits/s (check on https://testdebit.info/ )
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